Batwoman is a vigilante in Gotham City who acts as female counterpart to Batman. The Golden Age version was crime-fighter Kathy Kane who fought with her side-kick Batgirl and romantically pursued Batman. In the modern era her legacy is taken up by the army brat Kate Kane, a lesbian expelled from West Point. Like her Golden Age counterpart, Kate Kane's Batwoman is a wealthy socialite and has a sidekick; her cousin and former Teen Titan Flamebird. She has been involved with the Batman Family, Batman Incorporated, the Female Furies and Spyder.

Katherine Kane is a proud lesbian and has been romantically involved with several women, including Renee Montoya, Mallory Kimball and Maggie Sawyer.