The Gotham University was the main institution for higher education and research on Gotham City.
One of the most notorious criminals in Gotham was once a psychology professor at the Gotham U. He was Jonathan Crane, who was sacked from the institution after he employed ruthless methods to teach about the psychology of fear.
The G.U. was later target of a vicious attack by former professor Vilmer, who sought revenge against the institution and the headmaster for his sacking after unethical experiments. The institution was saved by the timely intervention of Batman and Robin. Much later, the laboratories of Gotham University were used as a trap to capture The Pi-Meson Man.
After several inmates from Arkham Asylum were released, Scarecrow used a large group of students at the Gotham University as his test subjects and using one of the building's classrooms, he transformed them into his minions. With a small army at his command, Scarecrow killed one student before leaving the place.
- Jonathan Crane (formerly)
- Stephanie Brown
- Jordanna Spence
- Francisco Gracia
- Michael Lane (formerly)
- Newton Flitwick (Deceased)
- Professor Vilmer (formerly)
- Gotham University Nighthawks (football)
The Gotham University was administered by Dr. Long, a man who became known for firing Jonathan Crane from the University staff. A few years later, Crane reappeared as the Scarecrow and attacked the University, stealing funds and releasing his fear toxin among the staff members. Crane was eventually stopped and captured by Batman.