The Gotham Zoo is the main zoo in Gotham City and house to several animal species.
After a zoo ship had an accident near the Gotham Harbor, some of the animals broke free and roamed the city. Batman and Robin rounded the animals and captured the resposible for the incident. All the animals were subsequently moved to the Gotham Zoo, including a rhino, a giant mole, a couple of condors, a great ape and a sloth.
Some time later, a criminal called John Marrow tried to hide in the Zoo and the Dynamic Duo followed him there. Marrow tried to eliminate Batman by opening the lions' cage, creating the perfect distraction that would allow him to escape. Batman however, confronted the lions and placed them back on the cages while Robin used a bat-bolo to capture Marrow.
Later, Batman and Robin captured a gang that was using the Zoo as their base of operations and hideout. A few years later, a couple of timber wolves were stolen from the Zoo by the criminal mastermind Achilles Milo.
Not long after this, the Zoo was used as hideout for the Riddler, who was hidden in the giraffes' area. Batman confronted Riddler in the Zoo and the fight ended with Riddler being tossed into the monkeys' cage. Afterwards, Batman started an investigation at the Zoo to clear his name and he was confronted by The Creeper. After a brief struggle, they worked together to stun some lions that were released by an unknown man and afterwards, they interrogated him, threatening to throw him at the aligators' cage. The man was murdered before he could reveal valuable information and the Zoo became the place where Batman and Creeper's alliance started.
Later, the zoo was the target of the arsonist Firefly. Batman arrived in time to stop Firelfy from burning the whole place down. Though he was attacked by some of the zoo's animals, Batman succeeded on capturing the criminal.